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초록

This study aimed at monitoring the acrylamide level of coffee due to the increasing coffee consumption as a favorite food. The acrylamide analysis was performed based on the internal standard method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) in coffee. For the test method validation, linearity, limit of detection(LOD), limit of quantification(LOQ), and accuracy have been tested. A 13C3-acrylamide was used as an internal standard in order to overcome the matrix effect. A total of 92 coffee and potato snack samples were collected from retail or coffee shops. The samples were cleaned up using an HLB solid-phase extraction(SPE) cartridge and Accucat SPE, then the LC-MS/MS measurement parameters were optimized for our objectives. The correlation coefficient(r2) of the calibration curve was 0.9999, and the limit of detection(LOD) and quantification(LOQ) were 0.97 and 2.93 μg/kg, respectively. The accuracy was tested using analysis reference material(RM: ground coffee), a