다시 뛰는 공정도시 서울!

초록

Cynanchum wilfordii Radix(Baekshuoh in Korean) is an important medicinal herb
plant used in the treatment of various ailments. Recently, it has been reported that C.
auriculatum, an adulterant of C. wilfordii, has been substituted for C. wilfordii in the
Korean herbal market. Due to the similarity of C. wilfordii and C. auriculatum, it is
hard to distinguish between them using an organoleptic test or histochemical methods.
In this study, we developed a Real-time PCR method using the psbA-trnH intergenic
spacer region. Among 90 samples purchased from markets and online from 2017∼2018,
C. auriculatum was detected in 20 samples(22.2%). This real-time PCR method using
novel primers based on sequence analysis of psbA-trnH was more sensitive relative to
conventional PCR methods, and compatible results were obtained using the matK gene.
These results indicate that real-time PCR analysis using a psbA-trnH genetic marker is
suitable for distinguishing between C. wilfordii and C. auri