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초록

Ochratoxin A(OTA) content was measured using liquid chromatography coupled
with tandem mass spectrometry(LC-MS/MS) and solid phase extraction column(SPE).
The quantitative analysis of LC-MS/MS can be affected by the matrix effect. The best
way to compensate for matrix effects is the use of the internal standard. 13C20-OTA was
used as an internal standard to normalize the matrix effect. Conditions for the sample
preparation and LC-MS/MS parameters were optimized for this purpose. A total of 80
wine and grape juice samples were collected from the retail or outlet markets; 52
samples were wine and 28 were grape juice. The analytical method was validated by
measuring samples fortified with OTA at various concentrations. Limits of detection
(LOD) and quantification(LOQ) were 0.09 and 0.28 g/kg, respectively. The recovery of
OTA spiked with wine and grape juice ranged 99.33∼107.22% and 91.80∼102.27%,
respectively. This optimized method was then used for the determination of OTA i